ZNF263

ZNF263
Identifikatori
AliasiZNF263
Vanjski ID-jeviOMIM: 604191 MGI: 1921370 HomoloGene: 4197 GeneCards: ZNF263
Lokacija gena (čovjek)
Hromosom 16 (čovjek)
Hrom.Hromosom 16 (čovjek)[1]
Hromosom 16 (čovjek)
Genomska lokacija za ZNF263
Genomska lokacija za ZNF263
Bend16p13.3Početak3,263,800 bp[1]
Kraj3,301,401 bp[1]
Lokacija gena (miš)
Hromosom 16 (miš)
Hrom.Hromosom 16 (miš)[2]
Hromosom 16 (miš)
Genomska lokacija za ZNF263
Genomska lokacija za ZNF263
Bend16 A1|16 2.2 cMPočetak3,561,957 bp[2]
Kraj3,568,654 bp[2]
Ontologija gena
Molekularna funkcija• GO:0001131, GO:0001151, GO:0001130, GO:0001204 DNA-binding transcription factor activity
• vezivanje sa DNK
• GO:0001948, GO:0016582 vezivanje za proteine
• vezivanje iona metala
• nucleic acid binding
• GO:0001078, GO:0001214, GO:0001206 DNA-binding transcription repressor activity, RNA polymerase II-specific
• sequence-specific DNA binding
• GO:0001200, GO:0001133, GO:0001201 DNA-binding transcription factor activity, RNA polymerase II-specific
Ćelijska komponenta• intracellular anatomical structure
• jedro
Biološki proces• GO:0009373 regulation of transcription, DNA-templated
• transcription, DNA-templated
• GO:1901227 negative regulation of transcription by RNA polymerase II
• GO:0045996 negative regulation of transcription, DNA-templated
Izvori:Amigo / QuickGO
Ortolozi
VrsteČovjekMiš
Entrez

10127

74120

Ensembl

ENSG00000006194

ENSMUSG00000022529

UniProt

O14978

Q8CF60

RefSeq (mRNK)

NM_005741

NM_148924

RefSeq (bjelančevina)

NP_005732

NP_683726

Lokacija (UCSC)Chr 16: 3.26 – 3.3 MbChr 16: 3.56 – 3.57 Mb
PubMed pretraga[3][4]
Wikipodaci
Pogledaj/uredi – čovjekPogledaj/uredi – miš

Cinkoprstni protein 263 je protein koji je kod ljudi kodiran genom ZNF263.[5] Gross (2014) mapirao je gen ZNF263 na hromosomu 16, sekvenca p13.3, na osnovu poravnanja sekvence ZNF263 (GenBank BC008805) sa genomskom sekvencom (GRCh37).[6]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 683 aminokiseline, a molekulska težina 77.299 Da.[7].

1020304050
MASGPGSQEREGLLIVKLEEDCAWSQELPPPDPGPSPEASHLRFRRFRFQ
EAAGPREALSRLQELCHGWLRPEMRTKEQILELLVLEQFLTILPQEIQSR
VQELHPESGEEAVTLVEDMQRELGRLRQQVTNHGRGTEVLLEEPLPLETA
RESPSFKLEPMETERSPGPRLQELLGPSPQRDPQAVKERALSAPWLSLFP
PEGNMEDKEMTGPQLPESLEDVAMYISQEEWGHQDPSKRALSRDTVQESY
ENVDSLESHIPSQEVPGTQVGQGGKLWDPSVQSCKEGLSPRGPAPGEEKF
ENLEGVPSVCSENIHPQVLLPDQARGEVPWSPELGRPHDRSQGDWAPPPE
GGMEQALAGASSGRELGRPKELQPKKLHLCPLCGKNFSNNSNLIRHQRIH
AAERLCMGVDCTEIFGGNPRFLSLHRAHLGEEAHKCLECGKCFSQNTHLT
RHQRTHTGEKPYQCNICGKCFSCNSNLHRHQRTHTGEKPYKCPECGEIFA
HSSNLLRHQRIHTGERPYKCPECGKSFSRSSHLVIHERTHERERLYPFSE
CGEAVSDSTPFLTNHGAHKAEKKLFECLTCGKSFRQGMHLTRHQRTHTGE
KPYKCTLCGENFSHRSNLIRHQRIHTGEKPYTCHECGDSFSHSSNRIRHL
RTHTGERPYKCSECGESFSRSSRLMSHQRTHTG
Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

Kloniranje i ekspresija

Sekvenciranjem cDNK nasumično odabranim iz biblioteke cDNK generirane iz granulocitoidnih ćelija koje su izvedene iz ćelijske linije 60 ljudske promijelocitne leukemije (HL60), Okubo et al. (1995) identificirali su djilomične cDNK koje predstavljaju nekoliko novih gena. Koristeći jednu od ovih cDNK, Yokoyama et al. (1997) izolirali su cDNK pune dužine koja kodira ZNF263, a koju su nazvali FPM315. Izvedeni protein od 683 aminokiseline sadrži devet C-terminalnih domena cinkovog prsta tipa C2H2, od kojih su neki povezani H/C vezama. Tu su i CRAB-kutija koja je u drugim ZNF-ima pokazala da djeluje kao represijski transkripcijski domen i N-terminalna regija bogata leucinom (LeR) koja je također bogata glutaminskim kiselinama, i visoko je konzervirana u podskupini ZNF-ova, a predviđa se da sadrži dva alfa-heliksa. Northern blot analizom otkrivena su glavna transkripta od približno 3,4 i 3,8 kb. ZNF263 je eksprimiran u svim ispitivanim ljudskim tkivima fetusa i odraslih.[8][9]

Reference

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000006194 - Ensembl, maj 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000022529 - Ensembl, maj 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ "Entrez Gene: Zinc finger protein 263". Pristupljeno 30. 1. 2017.
  6. ^ Gross, M. B. Personal Communication. Baltimore, Md. 6/25/2014.
  7. ^ "UniProt, O14978". Pristupljeno 11. 7. 2021.
  8. ^ Okubo, K., Itoh, K., Fukushima, A., Yoshii, J., Matsubara, K. Monitoring cell physiology by expression profiles and discovering cell type-specific genes by compiled expression profiles. Genomics 30: 178-186, 1995. PubMed: 8586417
  9. ^ Yokoyama, M., Nakamura, M., Okubo, K., Matsubara, K., Nishi, Y., Matsumoto, T., Fukushima, A. Isolation of a cDNA encoding a widely expressed novel zinc finger protein with the LeR and KRAB-A domains. Biochim. Biophys. Acta 1353: 13-17, 1997. PubMed: 9256059

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