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PRH2
Identifikatori
Aliasi	PRH2
Vanjski ID-jevi	OMIM: 168790 HomoloGene: 136785 GeneCards: PRH2
Lokacija gena (čovjek)
Hrom.	Hromosom 12 (čovjek)
Kraj	10,934,845 bp
Ortolozi
	5555
	n/a
	ENSG00000134551; ENSG00000275679; ENSG00000272803
	n/a
	P02810
	n/a
	NM_005042; NM_001110213
	n/a
	NP_001103683
	n/a
	Wikipodaci
Pogledaj/uredi – čovjek

Potporodica 2 prolinom bogatih proteina HaeIII su proteini koji su kod ljudi kodirani genom PRH2.^[3]

U jednoj studiji, proučavan je ljudski kiseli pljuvačni protein bogat prolinom PRP-1 i njegov C-terminalno skraćeni oblik PRP-3, analizom tandem-masenom spektrometrijom elektrozraka. Otkrivene su i okarakterizirane posttranslacijske modifikacije. Ostaci piroglutaminske kiseline su demonstrirani na N-terminalu, Ser-8 i Ser-22 su fosforilirani; identificirana je O-vezana konjugacija glukuronske kiseline. Postranslacijska modifikacija je locirana na Ser-17 i utvrđeno je da je prisutna u približno 40% polipeptida.^[4]

Kiseli proteini bogati prolinom (PRP) su dominantni proteini u pljuvaćki. Polimorfni su s više funkcija u stvaranju biofilma i urođenom imunosti. PRP-1 se sastoji od 150 ostataka, a PRP-3 je njegov C-terminalno krnji oblik 106 ostataka. N-terminalni domen sa 30 ostataka kiselih PRP-ova sadrži negativno nabijene aminokiseline i fosforilacije u Ser-8 i Ser-22. N-terminalno područje veže kalcij, posreduje adsorpciju u hidroksiapatit i regulira homeostazu kalcij-fosfata. C-terminalna sekvenca ProGln PRP-1 posreduje prianjanje na komensalne vrste gljiva Actinomyces i bakterija Streptococcus. Srednji domen bogat Pro-om podstiče vezivanje i inaktivaciju unesenih biljnih polifenola (tanina).^[4]

Nakon sekrecije, kiseli PRP -i brzo se obogaćuju na površinama zuba i razgrađuju se kao posljedica bakterijske proteolize. Nedavno je predloženo cijepanje kiselih PRP djelovanjem prolil endoproteaza komensalnih vrsta Streptococcus i Actinomyces za oslobađanje pentapeptida ArgGlyArgProGln, koji djeluje protiv proizvodnje laktata i desorbira povezane bakterije

Izlučeni proteini obično sadrže N– i O-vezane saharide. Ljudski kiseli PRP se smatraju neglikoziliranim proteinima, iako je zabilježena moguća glikozilacija. Nadalje, kiseli PRP iz pacovske pljuvačke, stimulirani β-adrenergičkim agonistima glikozilirani su, uglavnom glukuronskom kiselinom.^[4]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 166 aminokiselina, a molekulska težina 17.016 Da.^[5]

10	20	30	40	50
MLLILLSVAL	LAFSSAQDLD	EDVSQEDVPL	VISDGGDSEQ	FIDEERQGPP
LGGQQSQPSA	GDGNQDDGPQ	QGPPQQGGQQ	QQGPPPPQGK	PQGPPQQGGH
PPPPQGRPQG	PPQQGGHPRP	PRGRPQGPPQ	QGGHQQGPPP	PPPGKPQGPP
PQGGRPQGPP	QGQSPQ

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

Reference

^ ^a ^b ^c ENSG00000275679, ENSG00000272803 GRCh38: Ensembl release 89: ENSG00000134551, ENSG00000275679, ENSG00000272803 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Entrez Gene: Proline-rich protein HaeIII subfamily 2".
^ ^a ^b ^c Jonsson AP, Griffiths WJ, Bratt P, Johansson I, Strömberg N, Jörnvall H, Bergman T (juni 2000). "A novel Ser O-glucuronidation in acidic proline-rich proteins identified by tandem mass spectrometry". FEBS Letters. 475 (2): 131–4. doi:10.1016/s0014-5793(00)01645-8. PMID 10858503. S2CID 40612486.
^ "UniProt, P02810". Pristupljeno 13. 8. 2021.

Dopunska literatura

Jonsson AP, Griffiths WJ, Bratt P, Johansson I, Strömberg N, Jörnvall H, Bergman T (juni 2000). "A novel Ser O-glucuronidation in acidic proline-rich proteins identified by tandem mass spectrometry". FEBS Letters. 475 (2): 131–4. doi:10.1016/s0014-5793(00)01645-8. PMID 10858503. S2CID 40612486.
Bruno LS, Li X, Wang L, Soares RV, Siqueira CC, Oppenheim FG, Troxler RF, Offner GD (oktobar 2005). "Two-hybrid analysis of human salivary mucin MUC7 interactions". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1746 (1): 65–72. doi:10.1016/j.bbamcr.2005.08.007. PMID 16203048.
Schlesinger DH, Hay DI (april 1986). "Complete covalent structure of a proline-rich phosphoprotein, PRP-2, an inhibitor of calcium phosphate crystal growth from human parotid saliva". International Journal of Peptide and Protein Research. 27 (4): 373–9. doi:10.1111/j.1399-3011.1986.tb01030.x. PMID 3710693.
Wong RS, Hofmann T, Bennick A (juni 1979). "The complete primary structure of a proline-rich phosphoprotein from human saliva". The Journal of Biological Chemistry. 254 (11): 4800–8. PMID 438215.
Maeda N, Kim HS, Azen EA, Smithies O (septembar 1985). "Differential RNA splicing and post-translational cleavages in the human salivary proline-rich protein gene system". The Journal of Biological Chemistry. 260 (20): 11123–30. PMID 2993301.
Schlesinger DH, Hay DI (januar 1981). "Primary structure of the active tryptic fragments of human and monkey salivary anionic proline-rich proteins". International Journal of Peptide and Protein Research. 17 (1): 34–41. doi:10.1111/j.1399-3011.1981.tb01965.x. PMID 7228490.
Kim HS, Maeda N (maj 1986). "Structures of two HaeIII-type genes in the human salivary proline-rich protein multigene family". The Journal of Biological Chemistry. 261 (15): 6712–8. PMID 3009472.
Isemura S, Saitoh E, Sanada K (april 1980). "The amino acid sequence of a salivary proline-rich peptide, P-C, and its relation to a salivary proline-rich phosphoprotein, protein C". Journal of Biochemistry. 87 (4): 1071–7. PMID 7390979.
Hay DI, Bennick A, Schlesinger DH, Minaguchi K, Madapallimattam G, Schluckebier SK (oktobar 1988). "The primary structures of six human salivary acidic proline-rich proteins (PRP-1, PRP-2, PRP-3, PRP-4, PIF-s and PIF-f)". The Biochemical Journal. 255 (1): 15–21. doi:10.1042/bj2550015. PMC 1135184. PMID 3196309.

[refGRCh38Ensembl-1] ENSG00000275679, ENSG00000272803 GRCh38: Ensembl release 89: ENSG00000134551, ENSG00000275679, ENSG00000272803 - Ensembl, maj 2017

[2] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[entrez-3] "Entrez Gene: Proline-rich protein HaeIII subfamily 2".

[<"Saliva"-4] Jonsson AP, Griffiths WJ, Bratt P, Johansson I, Strömberg N, Jörnvall H, Bergman T (juni 2000). "A novel Ser O-glucuronidation in acidic proline-rich proteins identified by tandem mass spectrometry". FEBS Letters. 475 (2): 131–4. doi:10.1016/s0014-5793(00)01645-8. PMID 10858503. S2CID 40612486.

[UniProt-5] "UniProt, P02810". Pristupljeno 13. 8. 2021.

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PRH2

Aminokiselinska sekvenca

Reference

Dopunska literatura

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