IAEDANS

IAEDANS
Names
Preferred IUPAC name
5-{[2-(2-Iodoacetamido)ethyl]amino}naphthalene-1-sulfonic acid
Other names
5-[2-[(2-Iodo-1-oxoethyl)amino]ethylamino]-1-naphthalenesulfonic acid
1,5-IAEDANS
Identifiers
3D model (JSmol)
ChEBI
ChemSpider
ECHA InfoCard 100.048.418 Edit this at Wikidata
UNII
  • InChI=1S/C14H15IN2O4S/c15-9-14(18)17-8-7-16-12-5-1-4-11-10(12)3-2-6-13(11)22(19,20)21/h1-6,16H,7-9H2,(H,17,18)(H,19,20,21) checkY
    Key: ZMERMCRYYFRELX-UHFFFAOYSA-N checkY
  • InChI=1/C14H15IN2O4S/c15-9-14(18)17-8-7-16-12-5-1-4-11-10(12)3-2-6-13(11)22(19,20)21/h1-6,16H,7-9H2,(H,17,18)(H,19,20,21)
    Key: ZMERMCRYYFRELX-UHFFFAOYAI
  • O=S(=O)(O)c1cccc2c1cccc2NCCNC(=O)CI
Properties
C14H15IN2O4S
Molar mass 434.25 g·mol−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
checkY verify (what is checkY☒N ?)

IAEDANS is an organic fluorophore (fluorescent molecule).[1] It stands for 5-({2-[(iodoacetyl)amino]ethyl}amino)naphthalene-1-sulfonic acid. It is widely used as a marker in fluorescence spectroscopy.

1,5-IAEDANS has a peak excitation wavelength of 336 nm and a peak emission wavelength of 490 nm. The extinction coefficient of the dye is 5700. It is soluble in dimethylformamide (DMF) or buffer above pH 6 and reacts primarily with thiols.

The absorption spectrum IAEDANS overlaps well with the emission spectrum of tryptophan, making it useful as an acceptor in FRET experiments. It can also be used as a resonance energy donor to fluorophores such as fluorescein, Alexa Fluor 488, Oregon Green, and BODIPY FL.

References

  1. ^ Takashi, R; Duke, J; Ue, K; Morales, M.F (1976). "Defining the "fast-reacting" thiols of myosin by reaction with 1,5 IAEDANS". Archives of Biochemistry and Biophysics. 175 (1): 279–83. doi:10.1016/0003-9861(76)90509-9. PMID 133635.

This article about an organic acid is a stub. You can help Wikipedia by adding missing information.

Content Disclaimer

Informasi ini disarikan dari Wikipedia dan disajikan kembali untuk tujuan edukasi. Konten tersedia di bawah lisensi CC BY-SA 3.0. Kami tidak bertanggung jawab atas ketidakakuratan data yang bersumber dari kontribusi publik tersebut.

  1. The information displayed on this website is sourced in part or in whole from Wikipedia and has been adapted for the purpose of restating it. We strive to provide accurate and relevant information, however:
  2. There is no guarantee of absolute accuracy. Wikipedia is an open, collaborative project that can be edited by anyone, so information is subject to change.
  3. It is not intended to constitute professional advice. The content displayed is for informational and educational purposes only. For important decisions (e.g., medical, legal, or financial), please consult a professional.
  4. Content copyright. Wikipedia is licensed under the Creative Commons Attribution-ShareAlike License (CC BY-SA). This means that content may be reused with appropriate attribution and shared under a similar license.
  5. Responsible use. Any risk arising from the use of information from this website is entirely the responsibility of the user.