Affinity capture

Affinity capture is a technique in molecular biology used to isolate desired compounds based on their chemical properties and a solid substrate.[1] Commonly, plates out of solid materials such as glass are coated with various reagents to allow for covalent bonding of a capturing molecule such as an antibody. Afterwards, a solvent containing a desired compound for isolation is poured onto the plate, and the compound binds to the receptors on the plate (hence the capturing of the compound). Washing the plate and removing the desired compound completes the purification process.[2]

Applications

Affinity capture has been used to isolate proteins by means of binding a peptide sequence to the solid substrate, thus allowing for protein capture.[3] The process has also been examined for potential automation, but the unique circumstances for any given experiment may impede reproducibility.[4]

See also

References

  1. ^ Mikhailov, V. S.; et al. (January 2013). "Affinity capture of specific DNA fragments with short synthetic sequences". Russian Journal of Bioorganic Chemistry. 39 (1): 72–76. doi:10.1134/S106816201301007X. PMID 23844509. S2CID 254980916.
  2. ^ Johnson, Erin M.; et al. (1 August 2009). "Affinity capture mass spectrometry of biomarker proteins using peptide ligands from biopanning". Analytical Chemistry. 81 (15): 5999–6005. doi:10.1021/ac900868q. PMC 2846195. PMID 19572565.
  3. ^ LaCava, J; et al. (1 July 2016). "Protein Complex Purification by Affinity Capture". Cold Spring Harbor Protocols. 2016 (7) pdb.top077545. doi:10.1101/pdb.top077545. PMID 27371601.
  4. ^ Tran, John C.; et al. (2016). "Automated Affinity Capture and On-Tip Digestion to Accurately Quantitate in Vivo Deamidation of Therapeutic Antibodies". Analytical Chemistry. 88 (23): 11521–11526. doi:10.1021/acs.analchem.6b02766. PMID 27797494.


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