Rosalie married Ed Farley with whom she had 10 children.[3] The couple advocated for self-government of the Omaha people. They argued for, and prevailed in getting land allocated to individuals but retaining control of the unallocated land to the tribe rather than the state or federal government.[4][1][2] She and her husband advocated for autonomy when other family members advocated for "assimilation".[2] La Flesche Farley acted as business manager for the Omaha tribe when the land ownership negotiations were occurring. She was active in financial affairs of the individual members as well as the tribe as a group.[4]
La Flesche Farley died in 1900 at the age of 39.[1] The village of Rosalie, Nebraska is named for her.[4]