dnaC is a loading factor that complexes with the C-terminus of helicasednaB and inhibits it from unwinding the dsDNA at a replication fork.[1] A dnaB and dnaC associate near the dnaA bound origin for each of the ssDNA.[1] One dnaB-dnaC complex is oriented in the opposite direction to the other dnaB-dnaC complex due to the antiparallel nature of DNA. Because they are oriented in opposite directions, one dnaB-dnaC complex will complex with dnaA from the N-terminus of dnaB whereas the other dnaB-dnaC complex will complex with dnaA from the dnaC. After the assembly of dnaG onto the N-terminus of dnaB, dnaC is released and dnaB will be allowed to begin unwinding dsDNA to make room for DNA polymerase III to begin synthesizing the daughter strands.[1]
This interaction of dnaC with dnaB requires the hydrolysis of ATP.[2]
References
^ abcSlonczewski JL, Foster JW, Gillen KM (2009). Microbiology: an evolving science (1st ed.). New York: W.W. Norton & Co. ISBN978-0-393-97857-5.