The church's rector from 1862 to 1869 was Phillips Brooks, who was also the author of the lyrics to the familiar Christmas carol "O Little Town of Bethlehem". Renowned (and sometimes reviled) for his ebullient homilies and his staunch opposition to slavery, Brooks delivered a eulogy to Abraham Lincoln in the church on April 23, 1865, following the U.S. President's assassination on April 14. This sermon was reprinted and widely read. After the end of the American Civil War, Brooks took a sabbatical from the church to travel to Europe, Israel and Palestine. His visit to Bethlehem inspired him to write a poem for his Sunday School students, and for the church's 1868 Christmas season he had church organist Louis Redner set the poem to music. The result was “O Little Town of Bethlehem."
In 1869, Brooks became rector of Trinity Church, Boston, where he oversaw the design, decoration and construction of Trinity's new Back Bay, Boston facility from 1872 to 1877 following the original church's destruction in Boston's Great Fire of 1872.
The church's rector from 1899 to his death in 1932 was rev. Floyd W. Tomkins.
^Kirkbride, Mrs. Earle R. (1972). "Church of the Holy Trinity"(PDF). National Register of Historic Places. Pennsylvania Historical and MuseumCommission. Retrieved January 8, 2014.